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#2720 THERMAL CYCLER TOUCHDOWN PCR MANUAL#
#2720 THERMAL CYCLER TOUCHDOWN PCR SOFTWARE#

Make sure the Reporter Dye is ROX, and Quencher Dye is none. And of course, it provides the same reliability and performance that customers around the world have come to expect from Applied Biosystems thermal cyclers. Their ultrathin wall design will ensure rapid thermal transfer and a significant reduction in cycle and. If there is no predefined detector for this application, create a new one. The Applied Biosystems 2720 Thermal Cycler combines industry-standard technology from our GeneAmp® PCR System 9700but in a more compact package and at a lower price.

Easy optimization this gradient thermal cycler allows fast PCR optimization using a unique. Consistent results robust design ensures effective and consistent performance run to run. Intuitive touch screen easy programming using a large color touch screen and intuitive interface.

Create a new experiment, choosing Absolute Quantification assay type. Features and Benefits of the T100 Thermal Cycler.

For more details on the analysis, you can refer to this paper: “The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability.” Nat Protoc 2007 2(9):2212-21.įor an experiment using SDS v1.x, follow the directions below: In a thermal cycler (The Applied Biosystems 2720 Thermal Cycler).

#2720 THERMAL CYCLER TOUCHDOWN PCR SOFTWARE#

If you have the older software (v 1.x), you will have to program the software differently (see below) and analyze the results independently. 10 The genomic DNA was amplified using the Touchdown (TD) PCR assay11 and. When The Optimal Annealing Temperature Is Unknown, One Strategy, Touchdown.

#2720 THERMAL CYCLER TOUCHDOWN PCR MANUAL#

If you have the newer version of the software (v2.0.1 or later), which creates *.eds files, your data will be directly compatible with our Protein Thermal Shift™ Software. For PCR machine whose fluorescent signals is read at the top, Nest white PCR plates can effectively prevent the cross interference between different holes. Applied Biosystems GeneAmp PCR System 9700 Manual Online: touchdown pcr, Xl Pcr.

Gene expression, genotyping, copy number variation, HRM, protein thermal shift, protein detection, mutation detection, miRNA, presence/absence

Tungsten-halogen lamp, 5 excitation filters, 5 emission filters, CCD Cameraįor Research Use Only. Access tips and tricks related to programming and running of your PCR machine, including ProFlex, Veriti®, 2720, GeneAmp® PCR System 9700, and SimpliAmp PCR thermal cyclers. FAM™, SYBR®, VIC®, ROX™, NED™, TAMRA™ dyes (Cy®3, Cy®5, and Texas Red® dyes – 7500F only)įast or standard (7500 has standard mode only)